Qiagen rotor gene 6000 software
Rotor-Gene Q 5plex System. Real-time PCR cycler with 5 channels green, yellow, orange, red, crimson , laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training. Real-time PCR cycler and High Resolution Melt analyzer with 2 channels green, yellow plus HRM channel, laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits.
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels green, yellow, orange, red, crimson plus HRM channel, laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits.
Rotor-Gene Q 6plex Platform. Real-time PCR instrument with 6 channels blue, green, yellow, orange, red, crimson , including laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training not included.
Real-time PCR cycler and High Resolution Melt analyzer with 2 channels green, yellow plus HRM channel, laptop computer, software, accessories: includes includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits.
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels green, yellow, orange, red, crimson plus HRM channel, laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits. Rotor-Gene Q 6plex Priority Package. Real-time PCR instrument with 6 channels blue, green, yellow, orange, red, crimson , including laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits.
Real-time PCR cycler and High Resolution Melt analyzer with 2 channels green, yellow plus HRM channel, laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training. Real-time PCR cycler and High Resolution Melt analyzer with 5 channels green, yellow, orange, red, crimson plus HRM channel, laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training.
Real-time PCR instrument with 6 channels blue, green, yellow, orange, red, crimson , including laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits. Rotor-Gene Q 6plex System. Real-time PCR instrument with 6 channels blue, green, yellow, orange, red, crimson , including laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training.
Rotor-Gene Q 2plex Platform. Real-time PCR cycler with 2 channels green, yellow , laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training not included. Software CD, user guide. All pseudo-unknowns were correctly clustered according to genotype. Highly sensitive detection of methylated DNA. Precise real-time PCR analysis. Flexible formats. Unique rotary design for outstanding performance The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available see figure " Cross-section of the Rotor-Gene Q.
Cross-section of the Rotor-Gene Q. Q-Rex Software Q-Rex Software is operating and analysis operating and analysis software that drives Rotor-Gene Q hardware and facilitates experiment setup, data acquisition and basic and advanced analyses.
Fast and easy access to templates and recent experiments Drag-and-drop creation of new experiments Plug-ins to expand capacity for specific applications Audit trail for 21CFR part 11 compliance The software is suitable for use by the most novice researchers, while maintaining the highly complex data analysis functions required by advanced researchers.
Rotor-Gene Q Software The comprehensive Rotor-Gene Q software package supports all current state-of-the art real-time analysis procedures from basic to advanced algorithms. REST software is available for download under the "Resources" tab, and provides valuable analysis, including: Estimation of up- and down-regulation for gene expression studies Randomization and bootstrapping techniques Graphical data output via whisker-box plots Traditional relative quantification enables estimation of gene expression.
Further information is available from the following links: Rotor-Gene AssayManager v1. RGQ, Full Agreement. Instrument repair service for the Rotor-Gene Q at regional repair center. Shipping, labor and parts included during the Full Agreement period. Loaner instrument provided within 2—3 business days. Instrument repair turnaround time of 7—10 business days. Shipping, labor and parts costs included during the Full Agreement period.
RGQ, Core Agreement. One-time Rotor-Gene Q instrument repair performed at a regional repair center including shipping, labor, and spare parts for a period of one year. Resources Quick-Start Protocols 1. PDF 69KB. Supplementary Protocols 3. PDF KB. Scientific Posters 4. PDF 1MB. Scientific Poster EN. Instrument Technical Documents 3. PDF 76KB. Analysis Software For use with the Q-Rex Software v1. Q-Rex Basic Plug-in 2. For use with the Q-Rex Software v. Q-Rex Gene Expression Plug-in 2.
For use with Q-Rex Software version 1. For use with the Q-Rex Software version 1. This user manual provides information about the functions and features of the Q-Rex Melt Curve Analysis plug-in.
Q-Rex Target Detection Plug-in 1. Q-Rex Absolute Quantification Plug-in 2. Software User Guides 6. This user manual provides information about the functions and features of the Q-Rex End-Point Analysis plug-in. PDF 4MB. PDF 2MB. First, the target sequence is amplified by PCR to a high copy number.
Next, high-precision melting of PCR products enables discrimination of samples according to sequence, length, GC content, or strand complementarity, down to single base-pair changes.
No prior sequence information is needed, enabling detection of previously unknown and even complex sequence variations in a simple and straightforward way. The Rotor-Gene Q cycler has a unique rotary design that provides outstanding thermal and optical performance, making it ideal for use in HRM analysis.
HRM data analysis discriminates between genotypes by comparing the position and shape of melting curves of different samples. Melting curves of heterozygotes and homozygotes differ in their shapes and melting points T m. In standard HRM software packages, variations in melt curve shape and position compared to a control are used to differentiate between samples.
This method can cause unreliable, difficult-to-interpret results, and time-consuming manual data interpretation may be necessary. The software guides the user through all the steps, giving information about any choices that can be made at each step. In the first step in analysis, raw data are normalized by applying curve scaling to a line of best fit so that the highest fluorescence value is equal to and the lowest is equal to zero.
Next, the curves are differentiated and a composite median curve is constructed using the median fluorescence of all samples. The melt traces for each sample are subtracted from this composite median curve to draw a residual plot. The individual sample characteristics are extracted by principal component analysis from the residual plot. Principal component analysis is a well-established method of data analysis.
Principal component analysis highlights similarities and differences in the data and is used to create a cluster plot in supervised or unsupervised mode see figure " Identification of a class IV SNP.
Clustering grouping of data is performed according to allele. Supervised mode is often used for SNP genotyping, where the genotypes are known. In supervised mode, the user assigns one or more control samples for each cluster and the software classifies autocalls all unknown samples to clusters according to their characteristics.
The unsupervised mode is used to find new mutations in the data when there is no prior knowledge or only partial knowledge of the genotypes present in the samples.
In unsupervised mode, the software calculates the optimum number of clusters by itself. The recall specifically pertains to the Qiagen RGQ software version 2. According to the recall notice, when this software completes an export of data to a laboratory information management system LIMS , the comma-separate values. Qiagen issued an urgent medical device correction via email to customers on Jan. Four key elements have been added to create the ET software version; secure application login, database file management, electronic audit trail, and report generation.
Sacace - SyCycler 96 - A new instrument for real-time amplification and melting analysis suitable both for research studies as well as for diagnostics applications. Two thermoeletric Peltier elements ensure high accuracy of temperature regulation and noiselessness. The unique design and project of the thermal block allows loading and unloading of test samples in a completely automated software-controlled way. Mic is a small well qPCR instrument that uses magnetic induction technology for rapid real time cycling.
With an intuitive software layout, run setup and analysis become simple and there are many quality control features to provide confidence in the final result. The software has been designed to meet the MIQE guidelines.
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